CY3荧光染料用哪个波段激发?CY3发出的是什么光?

2018-05-08 12:57:11 570

CY3荧光染料用哪个波段激发?

经常有同学咨询,CY3荧光染料用哪个波长激发,发出来的是什么颜色的光,现将厂家的说明提供如下:

Invitrogen Cy3 dye is a bright, orange-fluorescent dye that can be excited using the 532 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. In addition to immunocytochemistry applications, Cy3 dye is commonly used to label nucleic acids.

The Cy3 fluorophore is also the basis for the lipophilic neuronal and long-term DiI cell tracing reagents, which append alkyl tails (≥12 carbons) onto the core fluorophore. The Cy3 dye is a traditional orange-fluorescent label for protein and nucleic acid conjugates used for imaging, flow cytometry, and genomic applications. It is also the basis for the classic lipophilic tracer DiI and its variants.

从上面资料可以看到,CY3荧光染料用532nm波段激发(绿光),发出来的是橙色光。

CY3的激发波长和发射波长

CY3的激发波长和发射波长

Photos of Streptavidin Cy3

Photos of Streptavidin Cy3

Cy3染料是一种明亮的橙色荧光染料,可以使用532nm的激光谱线,并通过TRITC(四甲基罗丹明)滤光片来显示。除了免疫细胞化学应用外,Cy3染料通常被用来标记核酸。Cy3属于氨基反应染料。分子式为:暂无数据。分子量为:668.86